Identification of soluble proteins present in giant squid (Dosidicus gigas) meal for human consumption

Introduction. The giant squid (Dosidicus gigas) is a species of great abundance and considered as potential resource to meet the demand for protein in Peru. Giant meal represents an alternative traditional proteins which can be used food fortification. Objective. To optimize extraction process identification soluble from (GSM). Materials methods. This study was conducted at Universidad Nacional Agraria La Molina, Lima, Peru, between 2018 2019. obtain highest yield (Ŷ) extracted, alkaline method using NaOH followed by acid precipitation pH 4.5 applied. Surface response methodology determine optimal parameters such temperature, concentration NaCl, time, ratio GSM: solvent. A 1D 2D electrophoresis carried out find distribution molecular weights identify main GSM. Results. Values were NaCl 0 M, time 35 min, solvent 1:31.72, temperature 71.9 ºC. detected range 6.5 38.37 kDa, would correspond tropomyosins, troponins, myosin light chain residues. Conclusions. allowed corresponding sarcoplasmic fraction (GSM), could industry.